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Animal models for vaccinia virus keratitis
Author(s) -
BRANDT C
Publication year - 2010
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2010.2212.x
Subject(s) - vaccinia , keratitis , virus , pathogenesis , cornea , virology , medicine , animal model , immunology , biology , dermatology , ophthalmology , biochemistry , gene , recombinant dna
Purpose Studies on pathogenic mechanisms involved in viral ocular infections and testing of potential therapies requires well established quantitative animal models. We present a new model of Vaccinia virus keratitis. Methods Rabbits were infected with the Dryvax strain of Vaccinia virus and disease was scored using a modified MacDonald‐Shadduck scoring system. The model will be described and examples of the use of the model for studies of pathogenesis and testing therapeutic approaches will be presented. Results The optimum dose of virus was determined (10e5 PFU). Infiltration of neutrophils followed predominantly by CD4+ cells occurred in the cornea and the optimum therapy was determined to be topical viroptic 9 times per day for 10 days. Steroid use should be avoided. Conclusion A new model of Vaccinia keratitis has been developed that is useful for pathogenesis and therapeutic studies.Commercial interest