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Neuroprotective effect of intravitreal cell‐based glucagon‐like peptide‐1 production in the optic nerve crush model
Author(s) -
Zhang Rong,
Zhang Haijuan,
Xu Liang,
Ma Ke,
Wallrapp Christine,
Jonas Jost B.
Publication year - 2011
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2010.02044.x
Subject(s) - retinal ganglion cell , optic nerve , retina , saline , retinal , superior colliculus , ganglion , ophthalmology , ganglion cell layer , medicine , chemistry , anatomy , biology , endocrinology , neuroscience
. Purpose: To examine the effect of intraocularly produced glucagon‐like peptide‐1 (GLP‐1) on the survival rate of retinal ganglion cells in an optic nerve crush model. Methods: Forty‐one Sprague–Dawley rats were divided into a study group (21 animals) in which 4 beads with 3000 genetically modified cells to produce GLP‐1 were intravitreally implanted into the right eye; a saline control group ( n = 12) with intravitreal saline injection; and a GLP‐1 negative bead control group ( n = 8) in which 4 beads with 3000 cells without GLP‐1 production were intravitreally implanted. The right optic nerves of all animals were crushed in a standardized manner. After labeling the retinal ganglion cells by injecting 3% fluorogold into the superior colliculus, the animals were sacrificed, and the ganglion cells were counted on retinal flat mounts. Results: The retinal ganglion cell density of the right eyes was significantly higher in the study group (median: 2081 cells/mm 2 ; range: 1182–2953 cells/mm 2 ) than in the GLP‐1 bead negative control group (median: 1328 cells/mm 2 ; range: 1007–2068 cells/mm 2 ; p = 0.002) and than in the saline control group (median: 1777 cells/mm 2 ; range: 1000–2405 cells/mm 2 ; p = 0.07). Correspondingly, the survival rate (ratio of retinal ganglion cell density of right eye/left eye) was significantly higher in the study group (median: 0.72; range: 0.40–1.04) than in the GLP‐1 bead negative control group (median: 0.44; range: 0.36–0.68; p = 0.003) and than in the saline control group (median: 0.56; range: 0.36–0.89; p = 0.03). Conclusion: Glucagon‐like peptide‐1 produced by intravitreally implanted cell beads was associated with a higher survival rate of retinal ganglion cells after an experimental optic nerve crush in rats.