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Topical levofloxacin 1.5% Is effective in reducing levofloxacin‐resistant MRSA and FQ‐resistant pseudomonas aeruginosa in keratitis models
Author(s) -
KOWALSKI RP,
ROMANOWSKI EG,
MAH FS,
SHANKS RMQ,
GORDON YJ
Publication year - 2009
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2009.4356.x
Subject(s) - levofloxacin , tobramycin , saline , medicine , pseudomonas aeruginosa , vancomycin , staphylococcus aureus , keratitis , ofloxacin , microbiology and biotechnology , antibiotics , ophthalmology , ciprofloxacin , anesthesia , biology , bacteria , gentamicin , genetics
Purpose We compared topical levofloxacin 1.5% (LEV) to standard therapies in reducing levofloxacin‐resistant Staphylococcus aureus (MRSA) and FQ‐resistant Pseudomonas aeruginosa (PA) in keratitis models. Methods Both corneas of 32 NZW rabbits were intrastromally injected with 1000 CFU of MRSA (LV MIC=32 μg/ml; van MIC=2 μg/ml). After 4 hours (onset), 8 rabbits were sacrificed and the corneas were homogenized for colony counts. 24 rabbits were divided into 3 topical treatment groups: 1) LEV, 2) vancomycin 5%, and 3) saline. Treatment consisted of drops every 15 minutes/5 hours (21 drops). One hour after treatment, the rabbits were sacrificed and the corneas were homogenized for colony counts. Similar to MRSA, 32 NZW rabbits were intrastromally injected with 1000 CFU of PA (LV MIC=32 μg/ml, tob=0.75 μg/ml). After 16 hours (onset), 8 rabbits were sacrificed and the corneas were homogenized for colony counts. 24 rabbits were divided into 3 topical treatment groups: 1) LEV, 2) tobramycin 1.4%, and 3) saline. Treatment consisted of drops every 15 minutes/1 hour and every 30 minutes/7 hours (19 drops). One hour after treatment, the rabbits were sacrificed and the corneas were homogenized for colony counts. The CFU data were analyzed non‐parametrically. Results LEV significantly reduced more MRSA than vancomycin 5%, and both significantly reduced more MRSA than the onset and saline control (p<0.05). LEV was as effective as tobramycin 1.4% in reducing PA, and both significantly reduced more PA than the onset and saline control (p<0.05). Conclusion LEV was more effective for reducing MRSA and as effective for reducing PA as standard therapies in rabbit keratitis models.Commercial interest