Premium
Oxidative stress from in vivo dual waveband exposure to ultraviolet radiation
Author(s) -
SÖDERBERG PG,
LI Y,
GALICHANIN K,
KRONSCHLäGER M
Publication year - 2009
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2009.3441.x
Subject(s) - confidence interval , in vivo , oxidative stress , additive function , spectral properties , radiation , irradiation , nuclear medicine , chemistry , physics , medicine , mathematics , optics , biology , biochemistry , computational chemistry , mathematical analysis , microbiology and biotechnology , nuclear physics
Purpose The purpose of the current project was to elucidate if the effect of exposures to two wavebands is directly proportional to the sum of the biologically efficient dose for each of the two wavebands. Methods Altogether 40 albino Sprague‐Dawley rats, 6 weeks old (150 g), were exposed unilaterally in vivo to UVR. Half the group was exposed to 8 kJ/m2 biologically efficient dose (= absolute dose) of UVR centered at 300 nm. The other 20 rats were exposed, first to 4 kJ/m2 biologically efficient dose (= absolute dose) of UVR centered at 300 nm, and then to 4 kJ/m2 biologically efficient dose (= 42 kJ/m2 absolute dose) centered at 310 nm. All animals were sacrificed at 1 week after exposure and intensity of forward light scattering was measured in both lenses. Results The UVR 300+310 nm group expressed slightly more light scattering than the UVR 300 nm only group as indicated by a 95 confidence interval for the difference approximating the degrees of freedom due to different variances (CI:D(0.95)= ‐8.69 +/‐6.92 x10‐2 tEDC, d.f = 32.7). Conclusion Although, the outcome indicated that there is a difference between the two groups, the difference is very small in relation to spectral sensitivity. It is therefore concluded that it is a valid approximation to assume spectral additivity of biologically efficient doses when estimating effects of broadband exposures.