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Challenges in optical measurement of scatter
Author(s) -
GINIS H
Publication year - 2009
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2009.3242.x
Subject(s) - optics , transparency (behavior) , computer science , human eye , diffraction , materials science , physics , computer security
Purpose Measurement of scattered light in biological media and particularly the optical media of the human eye is associated with two main challenges: The extremely low intensity of the scattered light and artefacts associated to tissue handling and preparation. Methods Our strategies in reducing the errors associated with the above mentioned challenges include the utilisation of properly designed illumination schemes and appropriate procedures and devices for excising and mounting the specimens. Results Appropriate techniques that incorporate precise knowledge of the optical PSF of the system may prove useful for the accurate measurement of scatter light. Diffraction through the utilisation of narrow (e.g. 0.5mm) diaphragms may prove useful for the broadening of the optical PSF (while preserving knowledge of its shape) and therefore allow optical measurements of a wider range of angles. Conclusion Optical measurements of scatter may prove useful not only for the macroscopic characterisation of the optical quality of the optical media of the eye but also for the extraction of information concerning the microscopic structure of the irregularities causing the loss of transparency.