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New insights into Meibomian gland function from gene expression experiments
Author(s) -
SCHIRRA F,
SEITZ B
Publication year - 2009
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2009.2334.x
Subject(s) - meibomian gland , kegg , dna microarray , biology , immune system , gene expression , lipid metabolism , microarray analysis techniques , gene , signal transduction , function (biology) , microrna , gene expression profiling , significance analysis of microarrays , microbiology and biotechnology , transcriptome , endocrinology , immunology , medicine , genetics , eyelid , surgery
Purpose The definitive goal of scientific work is to elucidate connections and understand associated functions. In that respect comprehensive, expression studies using microarrays appear to be supreme instruments. We strived to reveal mechanisms of meibomian gland control by sex steroids. Methods The gene expression of mouse meibomian gland under the influence of sex steroids is being presented based on own work group data along with results from literature work‐up. GeneSifter Software is used for analysis based on z‐score analysis and visualization of affected genes within known KEGG pathways. Results Androgens were activating, for instance, pyruvate metabolism, steroid biosynthesis, regulation of autophagy, PPAR signaling pathway, lipid‐related pathways but down‐regulating immune‐related pathways such as B and T cell receptor or Jak‐STAT signaling pathways. Estrogens mainly influenced genes related to lipid metabolism and immune factors. Conclusion The combination of gene expression microarrays and appropriate analysis software contributed significantly in starting to elucidate information on meibomian gland control.