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Treatment of ocular surface injuries by the transfer of limbal and mesenchymal stem cells growing on nanofibrous scaffolds
Author(s) -
HOLAN V,
ZAJICOVA A,
LENCOVA A,
POKORNA K,
SVOBODOVA E,
KRULOVA M
Publication year - 2009
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2009.205.x
Subject(s) - mesenchymal stem cell , electrospinning , biocompatibility , scaffold , nanofiber , biomedical engineering , chemistry , polymer , materials science , nanotechnology , pathology , medicine , composite material , organic chemistry
Purpose To use nanofibrous scaffolds to grow and transfer limbal stem cells (LSC) and mesenchymal stem cells (MSC) to treat ocular surface injuries in a mouse experimental model. Methods Nanofibrous scaffolds were prepared by electrospinning procedure from a polyamid 6/12 (PA6/12). This polymer was selected on the basis of stability of nanofibres in aqueous solutions, biocompatibility and the best properties as a matrix for the growth of LSC and LSC. LSC and MSC labeled with a vital fluorescent dye PKH26 and grown on nanofibrous scaffold were transferred on the damaged eye surface, where their seeding and surviving were monitored. The effects of transferred cells on local inflammatory reactions were assessed by Real‐time PCR. Results The morphology, growth properties and viability of LSC and MSC on PA6/12 nanofibres were comparable with those on plastic. Transfer of LSC and MSC growing on nanofibrous scaffolds on the damaged ocular surface significantly inhibited local inflammatory reactions and supported the healing process. Conclusion The nanofibers prepared from polymer PA6/12 represent a convenient scaffold for LSC and MSC growth and transfer to treat SC deficiencies and various eye surface injuries.