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Oxidative DNA damage in patients with cataract
Author(s) -
Ates Orhan,
Alp Hakan Hamit,
Kocer Ibrahim,
Baykal Orhan,
Salman İlknur A.
Publication year - 2010
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2009.01583.x
Subject(s) - malondialdehyde , lipid peroxidation , oxidative damage , 8 hydroxy 2' deoxyguanosine , medicine , deoxyguanosine , significant difference , dna damage , oxidative phosphorylation , oxidative stress , gastroenterology , dna oxidation , dna , biochemistry , chemistry
Acta Ophthalmol. 2010: 88: 891–895 Abstract. Purpose: This study examines the levels of oxidative damage in patients with cataract. Methods: Blood samples were collected from 60 patients with cataract and 60 age‐ and gender‐matched healthy individuals to measure 8‐hydroxy 2‐deoxyguanosine (8‐OHdG) and malondialdehyde (MDA) levels. Results: A significant difference was observed in leukocyte 8‐OHdG levels in patients with cataract in comparison with healthy persons (p < 0.001). Similarly, a significant difference was observed in plasma MDA levels in patients with cataract in comparison with healthy persons (p < 0.001). In addition, a significant correlation was found between levels of 8‐OHdG in leukocyte DNA and plasma MDA ( r = 0.859, p < 0.001). Conclusion: This study measured the oxidative DNA damage by measuring the 8‐OHdG in the leukocyte DNA in patients with cataract. In addition, the level of MDA – a marker for lipid peroxidation – was measured to determine lipid peroxidation.