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Structural and cellular diagnosis of ocular surface squamous neoplasia using in‐vivo confocal microscopy
Author(s) -
PARROZZANI R,
GHIRLANDO A,
CORTESE M,
MIOTTO S,
URBAN F,
MIDENA E
Publication year - 2008
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2008.6265.x
Subject(s) - histopathology , anisocytosis , in vivo , pathology , stroma , medicine , confocal , cytology , lesion , confocal microscopy , biology , immunohistochemistry , optics , physics , microbiology and biotechnology , anemia
Purpose Aim of this study was to analyze in‐vivo structural and cellular features of ocular surface squamous (OSS) neoplasia using clinical confocal microscopy (CM). Methods Ten consecutive cases of OSS neoplasia were investigated using in‐vivo corneo‐conjunctival CM (ConfoScan4, Nidek, Gamagori, Japan) with a 40X surface‐ noncontact objective. Data obtained using CM were also compared to standard histopathology or cytologic samples obtained by scraping technique. Results CM analysis of OSS neoplasia showed good agreement with correspondent tumor cytology or histopathology, revealing some structural and cellular features of the lesions, including: loss of the normal epithelium structure in 10 cases (100%), high‐reflective areas in 10 cases (100%), inhomogeneous cell density in 8 cases (80%), cells debris in 8 cases (80%), abrupt demarcation at the lateral edge of the lesion in 7 cases (70%), nodular zone of cell proliferation in 6 cases (70%), fibrovascular structures in 3 cases (30%), keratin pearls in 1 case (10%) and pre‐Bowman space involvement in 1 case (10%). No anterior stroma involvement was documented. Cells anisocytosis, pleocytosis and anisonucleosis were also documented in 9 of 10 tumors (90%). Conclusion In‐vivo CM seems a reliable, non‐invasive method useful for in‐vivo diagnosis of OSS neoplasia.