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Retinal changes after acute increase of the intraocular pressure in adult mice
Author(s) -
PINILLA I,
CUENCA N,
SALINASNAVARRO M,
FERNANDEZSANCHEZ L,
ALARCONMARTINEZ L,
GARCIAMARTIN E,
AVILESTRIGUEROS M,
VILLEGASPEREZ MP,
VIDALSANZ M
Publication year - 2008
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2008.578.x
Subject(s) - ophthalmology , intraocular pressure , erg , retinal , retina , medicine , electroretinography , anatomy , biology , neuroscience
Purpose To investigate the effects of increased IOP on the outer retina and its circuitry. Methods Adult male swiss mice received a mean of 75 laser impacts (532 nm wavelength, 300 mW power, 0.5 second duration, 100 µm diameter spot size) over the limbar and episcleral veins of the left eye. Intraocular pressure (IOP) was measured with the Tonolab tonometer prior to and at 12 h, and 1, 2, 8 or 20 wks after lasering. Full field ERG responses were recorded from both eyes prior to and 1, 2, 8 or 20 wks after lasering. Mice were processed at 3 (n=5), 8 (n=4) or 12 (n=4) wks after lasering. Cryostat retinal sections were analized by fluorescent confocal microscopy. Results Laser treatment induced an increase in mean IOP of 130% over baseline 12 hours after lasering but reached basal levels by 1 wk. Three wks after lasering, immunostaining with recoverin and transducin could not document any changes in the ONL, but both ON‐rod bipolar and horizontal cells had lost their dendritic processes in the OPL. Sprouting of horizontal and bipolar cell processes were observed into the ONL. Eight weeks and 12 weeks after lasering, mice with severe impairment of their ERG (disappearance of b‐wave) presented loss of photoreceptor cells. PKC showed morphologic changes of rod bipolar cells and Calbindin showed abnormal and diminished horizontal cells. Immunoreactivity for synaptophysin was decreased and no all the rod bipolar dendrites showed relationship with rod spherules. Conclusion Increased IOP by laser photocoagulation of the perilimbar and episcleral veins in swiss mice results in changes of retina cell morphology and impairs retinal circuitries.