Premium
Short‐term effect of topical dorzolamide hydrochloride on intrastromal corneal pressure "in vivo" in rabbit corneas
Author(s) -
BOLIVAR G,
TEUS M,
GUTIERREZ C
Publication year - 2008
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2008.574.x
Subject(s) - dorzolamide , in vivo , medicine , ophthalmology , cornea , intraocular pressure , topical anesthesia , anesthesia , biology , timolol , microbiology and biotechnology
Purpose To test if treatment with topical dorzolamide influences the intrastromal corneal pressure (ICP) "in vivo" in rabbit corneas. Methods Interventional, prospective study. Topical dorzolamide (Trusopt 2% eye drops; Merck Sharp & Dohme, Spain) was administered in 7 eyes of New Zealand male rabbits three times daily for three consecutive days, and then the ICP changes were recorded with a reusable blood pressure transducer connected to the mid peripheral cornea. The ICP was measured in the same manner in 7 eyes of New Zealand male rabbits without any topical treatment (control group). Results The ICP averaged ‐6.2±3.2 mmHg, ‐10±5.8 mmHg and ‐12.5±8.7 mmHg at 15, 30 and 45 minutes in the control group, respectively. In the study group (dorzolamide treated eyes), the ICP readings were 1.8±3.4 mmHg, ‐0.28±4.3 mmHg and ‐1.8±5.3 mmHg at the same time points, respectively. The differences in the ICP between both groups were statistically significantly different at all time points (p=0.004, p=0.005, and p=0.02, respectively). Conclusion ICP is a valid and sensitive method to evaluate “in vivo” the endothelial function, and that it is more sensitive that other methods in detecting changes in the physiology of this structure with the use of topical dorzolamide.