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Impact of culturing on metabolic profile of human corneas
Author(s) -
KRYCZKA T,
EHLERS N,
MIDELFART A
Publication year - 2008
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2008.5335.x
Subject(s) - magic angle spinning , metabolic activity , andrology , ophthalmology , cornea , pathology , chemistry , biology , medicine , nuclear magnetic resonance spectroscopy , physiology , organic chemistry
Purpose The aim of this study was to examine possible differences in the metabolic profile between cultured and non‐cultured corneas. Methods Corneas from 12 donors were obtained post‐mortem and introduced to the tissue culture for 8‐20 days. The control corneal tissues were excised during enucleating of eyes with malignant melanoma in the retina from 4 patients and immediately frozen at ‐80 ºC. The metabolic profiles of the samples were investigated with HR MAS (High Resolution Magic Angle Spinning) 1H NMR (Nuclear Magnetic Resonance) spectroscopy (14.1 T) operating at 600.132 MHz. All data obtained with both methods were analysed using special software for: (i) analysis of complex mixtures, (ii) principal component analysis – PCA, (iii) detailed statistical analysis. Results Significant deferences in metabolic profiles between cultured and non‐cultured corneas were detected. The levels of several metabolites in cultured tissues were increased in comparison to the control. It was also shown that the levels of some of metabolites in samples cultured for 9‐14 days differed significantly from the samples kept for less than 9 days or 15‐20 days. Conclusion Corneal culturing results in the changes of the metabolic profile in tissue. The increase of the levels of the metabolites in organ within the second week of culturing may indicate increased enzymatic activity or/and increased response of cells toward stress factors in the tissue.