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Chemokines in proliferative diabetic retinopathy and proliferative vitreoretinopathy
Author(s) -
ABU EL ASRAR AM,
STRUYF S,
KANGAVE D,
GEBOES K,
VAN DAMME J
Publication year - 2008
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2008.5323.x
Subject(s) - proliferative vitreoretinopathy , diabetic retinopathy , cxcr3 , chemokine , medicine , vitrectomy , cxc chemokine receptors , retinopathy , chemokine receptor , angiogenesis , epiretinal membrane , neovascularization , fibrosis , immunology , inflammation , pathology , ophthalmology , endocrinology , diabetes mellitus , visual acuity
Purpose To determine levels of the chemokines I‐309, MCP‐1, MIP‐1α, MIP‐1β, MCP‐3, MCP‐2, ENA‐78, GCP‐2, IP‐10 and I‐TAC in vitreous and serum from patients with proliferative diabetic retinopathy (PDR), proliferative vitreoretinopathy (PVR) and retinal detachment with no PVR (RD) and expression of MCP‐1, SDF‐1 and the chemokine receptor CXCR3 in epiretinal membranes. Methods Vitreous and serum samples were obtained from 57 RD, 32 PVR and 88 PDR patients. The levels of chemokines were measured by ELISAs. Epiretinal membranes were studied by immunohistochemistry. Results MCP‐1 and IP‐10 were the only chemokines detected in vitreous. Levels and incidence of detection in vitreous were significantly higher than that in serum for MCP‐1 (p<0.001 for both comparisons) and IP‐10 (p=0.0035; <0.001, respectively). Levels were significantly higher in vitreous from patients with PVR and PDR compared with RD for MCP‐1 (p=0.0002) and IP‐10 (p=0.0083). Incidence of IP‐10 detection was significantly associated with increased levels of MCP‐1 in vitreous (p<0.001). MCP‐1, SDF and CXCR3 were expressed by myofibroblasts and vascular endothelial cells in membranes. Conclusion MCP‐1, IP‐10 and SDF‐1 may participate in pathogenesis of PVR. Clinical Relevance: Chemokines and their receptors could be molecular targets for preventing angiogenesis / fibrosis in the eye.