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Systemic inflammatory response after ocular in vivo UVR‐300 nm exposure
Author(s) -
MEYER L,
WEGENER A,
LÖFGREN S,
GALICHANIN K,
HOLZ FG,
SÖDERBERG PG
Publication year - 2008
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2008.5145.x
Subject(s) - in vivo , inflammatory response , medicine , infiltration (hvac) , pathogenesis , inflammation , tumor necrosis factor alpha , ophthalmology , pathology , biology , physics , microbiology and biotechnology , thermodynamics
Purpose To investigate if unilateral in vivo UVR‐B exposure of one eye affects the contralateral eye in a co‐cataractogenic, sympathetic reaction and to determine if an inflammatory response is involved in the pathogenesis. Methods C57BL/6 mice were unilaterally exposed in vivo to UVR‐B for 15 minutes. Groups of 24 animals each received 0x/ 2x/ 3x/ or 4x cataract threshold equivalent dose. 48 hours following UVR ‐ B exposure cataract morphology was documented in dark field illumination photography and light scattering was quantified, in both lenses in vitro. Serum levels of pro‐inflammatory cytokines IL‐1ß, IL‐6 and TNF‐α were analyzed with ELISA. Immunohistochemistry was performed for inflammatory infiltration in exposed and contralateral eyes. Results UVR‐B exposure induced cataract in all exposed lenses. There was additionally a significant UVR dose dependent increase of light scattering in contralateral not exposed lenses. Inflammatory infiltration was detected immunohistochemically in the anterior segment of both eyes. IL‐1β serum concentration increased with increasing UVR‐B exposure dose. There was a similar trend for serum IL‐6 but not for TNF‐α. Conclusion Unilateral UVR‐B exposure increases light scattering also in the contralateral eye and triggers a systemic inflammatory response mediated by IL‐1β and possibly IL‐6. Age related cataract is almost exclusively a bilateral event. Since a systemic inflammatory response might be an important factor in cataractogenesis our results might initiate new strategies in the prevention of the disease.