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Modifications in conjunctival and limbar epithelium after micro‐incision cataract surgery (MICS). Impression cytology study
Author(s) -
ALIO SANZ JL,
RODRIGUEZ AE,
WALEWSKA A,
FERRER C,
RODRIGUEZPRATS JL,
BATAILLE L
Publication year - 2008
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2008.467.x
Subject(s) - phacoemulsification , conjunctiva , medicine , goblet cell , cataract surgery , ophthalmology , population , cytology , surgery , pathology , epithelium , visual acuity , environmental health
Purpose To know if the exposition of the conjunctiva during the micro‐incision cataract surgery (MICS) and the energy released during the phacoemulsification produce alterations in the goblet cell population and nucleus / cito plasm relation in the different areas of the conjunctiva of these patients. Methods 32 patients scheduled for MICS surgery were included in this study. The cataract grade, phacoemulsification power, phacoemulsification time and time of the exposition of the conjunctiva were registered during the surgery. Goblet cell count, epithelial cell morphology and inflammatory cells were studied by conjunctival impression cytology before and after the surgery, at one and three months. The conjunctival samples were obtained from the nasal area, temporal area, and the phacoemulsification incision area. Results Mean age of the patients was 67‐years old. Mean phacoemulsification power was 6 %, and the “phaco” time was 30 seconds. Mean time of the exposition of the conjunctiva was 9 minutes and 30 seconds. Analysis by impression cytology of the ocular surface showed a goblet cell population pre‐operative of 408/mm2 in the nasal area, 317/mm2 in the temporal area, and 343/mm2 in phacoemulsification incision area. Goblet cell count in the three different places decreased significantly after MICS surgery: p=0.038, p=0.018, and p=0,001 respectively. Conclusion Impression cytology showed a significant reduction in the goblet cell populations after MICS in the three studied areas of the conjunctiva.