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Effect of different antioxidants in experimental alcoholic optic neuropathy
Author(s) -
MENEZO ROZALEN JL,
AVINOMARTINEZ JA,
PERISMARTINEZ C,
ESPANAGREGORI E,
ROMERO FJ
Publication year - 2008
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2008.423.x
Subject(s) - ebselen , malondialdehyde , lipid peroxidation , chemistry , glutathione , glutathione peroxidase , ethanol , oxidative stress , antioxidant , endocrinology , medicine , pharmacology , biochemistry , enzyme
Purpose To establish the possible beneficial effects of three synthetic antioxidants: S‐adenosil‐L‐methionine (SAMe), N‐acetyl‐L‐cysteine (NAC) and Ebselen in optic nerve damage following chronic ethanol feeding in adult rats. Methods Twenty male Sprague‐Dawley rats (five groups of four animals each) were fed a liquid, ethanol‐containing diet with littermates given isocaloric amounts of ethanol‐free diet serving as controls. Antioxidants were administered by dissolving them in the liquid diet at 0.4 mg/ml (SAMe), 0.163 mg/ml (NAC) and 5.33 mg/ml (Ebselen). After 6 weeks, optic nerves were obtained and parameters that are relevant for the modulation of oxidative stress, such as antioxidants ‐GSH content and Glutathione‐peroxidase (GSH‐Px) activity‐ and lipid peroxidation‐derived end products ‐malondialdehyde (MDA)‐ were determined. Comparisons between groups were done using Student´s t‐test for grouped data and one‐way ANOVA. Differences were considered significant at p<0.05. Results N‐acetyl‐L‐cysteine and Ebselen were able to normalise MDA content and to restore GSH content and GSH‐peroxidase activity, to values not significantly different from those of controls (p>0.05). Treatment with SAMe slightly improved values of some parameters, although no statistically beneficial (p<0.05 versus control). Conclusion These results support the use of NAC and Ebselen for the treatment of chronic alcohol‐induced optic nerve damage.