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INHIBITION OF CYTOCHROME OXIDASE AND BLUE ‐ LIGHT DAMAGE IN RAT RETINA
Author(s) -
Chen Enping
Publication year - 1993
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1993.tb08724.x
Subject(s) - cytochrome c oxidase , darkness , retina , retinal , outer nuclear layer , cytochrome , chemistry , blue light , biophysics , rhodopsin , oxidase test , cytochrome c , biochemistry , biology , mitochondrion , enzyme , botany , neuroscience , optics , physics
. The activity of cytochrome oxidase, outer nuclear layer thickness and edema were quantitatively evaluated in the blue‐light‐exposed rat retina. Dark‐adapted or cyclic‐light‐reared rats were exposed to blue light with a retinal dose of 380 kJ/m 2 . Immediately, one, two, and three day(s) after exposure, the retinas of six rats from each adaptation group were examined. There was no difference between the dark‐adapted and cyclic‐light‐reared rats. Immediately after light exposure, cytochrome oxidase activity decreased. The activity in the inner segments remained low at day one, while severe edema was observed in the inner and outer segments. The outer nuclear layer thickness decreased one to three days after exposure. The blue‐light exposure inhibited cytochrome oxidase activity and caused retinal injury. Similarity of the injury process in the dark‐adapted and cyclic‐light‐reared retinas suggests that rhodopsin was not involved. The inhibition of cytochrome oxidase could be a cause of retinal damage.