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Microradiographical Determination of the Dry Mass Density in the Rat Retina 1
Author(s) -
Chen Enping,
Chung JangHyun,
Söderberg Per G.,
Lindström Bo
Publication year - 1993
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1993.tb08720.x
Subject(s) - retina , retinal , dry weight , resolution (logic) , optical density , chemistry , materials science , optics , physics , biology , botany , artificial intelligence , computer science , biochemistry
. A method for the quantitative measurement of the dry mass density in retinal tissue is presented. Cryosections of rat retinas were freeze‐dried and exposed to soft X‐rays together with a reference system. The section thickness was measured optically. The X‐ray energy absorbed by the retinal tissue was recorded photographically and the microradiographs were evaluated photometrically. These measurements were used to estimate the dry mass density for each retinal layer. Sources of variation were evaluated. The precision was found adequate. The present method allows a localized determination of the dry mass density and of the hydration in the retina at a microscopic resolution.