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Intracellular pH regulation by a Na + /H + exchanger in cultured bovine trabecular cells
Author(s) -
Chu TehChing,
Keith Charles,
Green Keith
Publication year - 1992
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1992.tb04886.x
Subject(s) - intracellular ph , chemistry , bicarbonate , amiloride , ouabain , dids , intracellular , hydrogen peroxide , nuclear chemistry , sodium–hydrogen antiporter , biophysics , sodium , chromatography , biochemistry , membrane , biology , organic chemistry
Intracellular pH (pH i ) of cultured bovine trabecular cells was measured using video‐imaging techniques with a pH‐sensitive intracellular fluorescent dye, BCECF. In bicarbonate‐rich Ringer at pH 7.4, pH i was 7.29 ± 0.03 (± SEM, n = 12 monolayers, 120 cells sampled). Exposure to 20 mM NH 4 Cl immediately alkalinized pH i : replacement with a Na + ‐rich solution acidified pH i before recovery to resting levels. When NH 4 Cl was replaced by a low Na + solution, acidification was sustained but pH i recovery occurred after Na + ‐rich solution. A pH i of 7.11 ± 0.02 (n = 2 monolayers, 20 cells) occurred in pH 6.8 and pH i was 7.72 ±0.03 (n = 2 monolayers, 20 cells) in pH 8.0. Amiloride (1 mM) acidified pH i but DIDS (1 mM) treatment, HCO 3 − ‐free condition, 1 mM ouabain, 50 mM K + , and 2 mM BaCl 2 failed to change pH i . Hydrogen peroxide (1 mM) acidified pH i but no change occurred with 50 μM. Trabecular cells possess an Na + /H + exchanger similar to that in other cell types.