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Immunohistochemistry of the monkey retina with a monoclonal antibody against subunit V of cytochrome c oxidase
Author(s) -
Isashiki Yasushi,
Nakagawa Masanori,
Higuchi Itsuro
Publication year - 1991
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1991.tb04822.x
Subject(s) - cytochrome c oxidase , immunohistochemistry , microbiology and biotechnology , monoclonal antibody , retina , inner plexiform layer , chemistry , western blot , biology , protein subunit , antibody , biochemistry , enzyme , immunology , neuroscience , gene
A monoclonal antibody against subunit V of cytochrome c oxidase was newly prepared from purified bovine heart muscle enzyme. This antibody showed cross‐reactivity against the bovine and the human cytochrome c oxidase on Western blot. Cytochrome c oxidase in paraffin embedded sections of monkey retinas and optic nerves was examined immunohistochemically using the monoclonal antibody. The inner segment of cone and rod photoreceptors, the inner nuclear layer and the inner plexiform layer showed marked reactivities. The optic nerve fiber axons also showed high reactivities, with more in the unmyelinated fibers peripheral to the lamina cribrosa. These immunohistochemical results using the antibody are mostly agreeable with previously reported histochemical localization of cytochrome c oxidase activity in the retina, but there were some noticeable differences in the plexiform layers.