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Residual H 2 O 2 compromises deswelling function of in vivo rabbit cornea
Author(s) -
Yuan Nanyong,
Pitts Donald G.
Publication year - 1991
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1991.tb02718.x
Subject(s) - cornea , aqueous solution , chemistry , aqueous humor , in vivo , rabbit (cipher) , ophthalmology , lagomorpha , nuclear chemistry , medicine , biology , mathematics , endocrinology , statistics , microbiology and biotechnology
. The purpose of the study was to investigate the effect of H 2 O 2 on corneal swelling, corneal deswelling and to determine the concentration of H 2 O 2 in the aqueous humor. Four types of experiments were performed using the rabbit eye. 1. Central corneal thickness (CCT) measurements after instillation of H 2 O 2 into the cul‐de‐sac and after wearing H 2 O 2 soaked soft contact lenses (SLC) for 2 h using 60 ppm, 100 ppm and 300 ppm H 2 O 2 . 2. Measurement of corneal deswelling induced by wearing SCL and closed eye for 2 h. 3. Determining the H 2 O 2 in the aqueous humor one h after instillation of H 2 O 2 into the cul‐de‐sac. CCT increases of 7.5% and 10.7% were found after the instillation of 100 ppm and 300 ppm H 2 O 2 into the cul‐de‐sac. After wearing H 2 O 2 soaked SCLs, CCT increased 4.6% and 5.8%, respectively. The H 2 O 2 in the aqueous humor was 0.087 mM or a 160% increase after using 300 ppm H 2 O 2 . The increase in CCT and interference with the deswelling process is interpreted as a toxic effect on the cornea and an interference with the normal dehydration function of the endothelial pump.