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Regeneration of the rat corneal epithelium after injury
Author(s) -
Sandvig Kjell U.,
Haaskjold Erling,
Bjerknes Rolf
Publication year - 1991
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1991.tb02049.x
Subject(s) - regeneration (biology) , mitotic index , corneal epithelium , cornea , epithelium , heptanol , mitosis , anatomy , ophthalmology , medicine , biology , pathology , microbiology and biotechnology , intracellular , gap junction
Central corneal epithelial defects with diameter 3.5 mm were made with n‐heptanol in the right eyes of rats. Erosions of the same size were made by mechanical scraping in the left eyes of the same animals. All the erosions were covered by epithelium after one day. After one day the mitotic rate and the labelling index were higher in the n‐heptanol treated corneas compared to the mechanically abraded ones. Both methods produced similar mitotic rates and labelling indexes after 3 and 12 days. The results from wound healing studies using n‐heptanol or mechanical scraping are therefore not entirely comparable because of the different regenerative responses after one day. However, the n‐heptanol method is easier to perform and to standardize, and is therefore preferable.