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Endothelial repair of posterior corneal wounds in rabbits. Influence of foetal calf serum in the culture medium
Author(s) -
Medin Helge,
Davanger Martin
Publication year - 1990
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1990.tb01903.x
Subject(s) - organ culture , endothelium , corneal endothelium , endothelial stem cell , ophthalmology , descemet membrane , wound healing , anatomy , medicine , pathology , cornea , chemistry , surgery , in vitro , endocrinology , biochemistry
The endothelial covering of experimental wounds on the posterior corneal surface has been studied in organ culture experiments. Defects involving the Descemet's membrane were produced, in addition to pure endothelial defects. The influence of foetal calf serum (FCS) on the healing process was examined. Wounds including the Descemet's membrane, about 1–2 × 3 mm, were not covered by cells after 12 days in FCS free medium. Such wounds were covered by endothelium after 6 days when cultivated in medium containing 10% FCS, and partly covered in 1% FCS. Pure endothelial defects, 4 mm in diameter, were cell‐covered within 3–4 days irrespective of the presence of FCS. Conclusion: the presence of FCS is required for the healing of defects involving the Descemet's membrane, and not required for the healing of pure endothelial defects.