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Fluorescein and fluorescein glucuronide in vitreous: fluorescence and binding properties in vitro
Author(s) -
Larsen Michael,
LundAndersen Henrik
Publication year - 1989
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1989.tb00742.x
Subject(s) - fluorescein , sodium fluorescein , fluorescence , sodium hyaluronate , chemistry , quenching (fluorescence) , in vitro , fluorescence spectroscopy , chromatography , biochemistry , optics , physics
. The total and ultrafiltrable fluorescence were measured after disodium fluorescein and fluorescein monoglucuronide were added to aliquots of human and porcine vitreous, and to aliquots of phosphate buffer with and without 0.2% sodium hyaluronate. No significant binding or quenching of F or FG by vitreous or sodium hyaluronate could be found, indicating that vitreous binding is not a source of error in vitreous fluorometry.