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T‐lymphocyte subsets in the human lacrimal gland
Author(s) -
Gudmundsson Olafur G.,
Benediktsson Hallgrimur,
Olafsdottir Kristrun
Publication year - 1988
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1988.tb08528.x
Subject(s) - lacrimal gland , immunoperoxidase , cytotoxic t cell , monoclonal antibody , inducer , biology , pathology , t lymphocyte , microbiology and biotechnology , antibody , immunology , medicine , antigen , in vitro , biochemistry , gene
. This study examines the microscopical appearance, location, distribution, subdivision and density of T‐lymphocytes in the human lacrimal gland. Fourteen glands, 7 from either sex, were removed and frozen shortly after the donors' death, and processed for immunoperoxidase staining, utilizing a biotinavidin system and one of the following monoclonal antibodies: Anti‐Leu‐1 and ‐Leu‐4, which recognize T‐lymphocytes, anti‐Leu‐2a, which binds to suppressor/cytotoxic T‐cells, and anti‐Leu‐3a+3b, which recognizes helper/inducer cells. The T‐lymphocytes in the human lacrimal gland were small to medium sized, mainly located in the interacinar tissue, often adjacent to an acinus or close to a collecting duct. The median number of each T‐cell subset per defined field (0.086 mm 2 ) at x 500 magnification was as follows: Males: Leu‐ 1 : 3.1, Leu‐2a: 4.9, Leu‐3a + 3b: 2.8, Leu‐ 4 : 4.5. Females: Leu‐ 1 : 4.3, Leu‐2a: 5.0, Leu‐3a + 3b: 3.7 and Leu‐ 4 : 5.8. The sex difference was not statistically significant. The helper/suppressor cell ratio in the human lacrimal gland was 0.57 for males and 0.74 for females.