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Effect of multiple applications of compound 48/80 on mast cells of rat conjunctiva
Author(s) -
Allansmith Mathea R.,
Baird Robert S.,
Ross Robert N.,
Barney Neal P.,
Bloch Kurt J.
Publication year - 1987
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1987.tb07015.x
Subject(s) - degranulation , compound 48/80 , conjunctiva , mast cell , mast (botany) , medicine , pharmacology , active compound , immunology , chemistry , stereochemistry , receptor
Abstract We sought to determine the effect on rat conjunctival mast cells of chronic exposure to a degranulating agent. Compound 48/80 (250 μg in 10 μl) was applied daily to the ocular surface of rats for a total of 13 treatments administered in 17 days. A single application of compound 48/80 was given to rats for comparison. Four groups of animals (6 in each group) were evaluated 1 or 48 h after the last application of compound 48/80 in both chronic exposure and single exposure studies. Although a single exposure to compound 48/80 induced significant degranulation of mast cells in animals observed 1 h after treatment, no significant degranulation of mast cells was observed 1 h after the last dose of compound 48/80 was given to rats whose conjunctiva had been chronically exposed to this degranulating agent. Thus, multiple applications of compound 48/80 attenuated the mast cell response to compound 48/80. This acquired tolerance to compound 48/80 was reflected in the lessened clinical signs in rats receiving repeated applications compared with those given a single application.