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Use of tear enzyme activities to assess the corneal response to contact lens wear
Author(s) -
Fullard Roderick J.,
Carney Leo G.
Publication year - 1986
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1986.tb06903.x
Subject(s) - contact lens , lactate dehydrogenase , corneal epithelium , malate dehydrogenase , ophthalmology , lens (geology) , effects of long term contact lens wear on the cornea , in vivo , cornea , enzyme , chemistry , medicine , biology , biochemistry , microbiology and biotechnology , paleontology
A non‐invasive biochemical method for assessing the effects of contact lens wear on the in vivo corneal epithelium is described. A fluorometric technique is used to measure the activities of lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) in human tear fluid. In view of the corneal epithelial origin of these enzymes, changes in their activity can indicate the severity of environmental stresses on the corneal epithelium. Following short‐term wear of contact lenses, LDH and MDH activities are altered so that the tear LDH/MDH ratio is elevated. The magnitude and time course of this elevation are influenced by contact lens type, fit and duration of wear. This technique can yield more specific measures of the corneal response to contact lens wear than previous techniques.