Premium
THE HEALING OF RABBIT CORNEAL ENDOTHELIUM
Author(s) -
OLSEN ERLING GRØNVOLD,
DAVANGER MARTIN
Publication year - 1984
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1984.tb05808.x
Subject(s) - corneal endothelium , cornea , rabbit (cipher) , cell division , endothelium , in vivo , anatomy , cell , endothelial stem cell , hexagonal crystal system , in vitro , wound healing , chemistry , microbiology and biotechnology , ophthalmology , pathology , biology , medicine , surgery , endocrinology , biochemistry , crystallography , statistics , mathematics
A circular 4 mm endothelial defect was induced by transcorneal freezing. The experimental damage and the healing took place in the living rabbit in 15 eyes, and in the isolated cornea in organ culture in further 20 eyes. The reparative process was studied by SEM, and proved to be the same in vivo and in vitro. The defect was covered with endothelial cells after 3 days. The normal hexagonal pattern was regained after 3 weeks. Both cell migration and cell division were involved in the reparative process. Only cells recruited from a zone close to the defect were active; the cells situated more than a few cell diameters from the original edge maintained their form and size unchanged. The first phase of cell division was the formation of a spherical cell with numerous blebs on its surface.