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HUMAN CORNEAL ENDOTHELIAL CELL DENSITY AFTER AN IN VITRO IMMITATION OF ELEVATED INTRAOCULAR PRESSURE
Author(s) -
MORTENSEN ANDERS CHR.,
SPERLING STEFFEN
Publication year - 1982
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1982.tb03041.x
Subject(s) - intraocular pressure , ophthalmology , cornea , in vitro , cadaver , elevated intraocular pressure , endothelial stem cell , chemistry , medicine , anatomy , biochemistry
Normal human cadaver corneas were mounted in test chambers 10–52 h post mortem, regenerated for 24 h in a modified tissue culture medium, and exposed to a laboratory immitation of elevated intraocular pressure. The endothelial cell density was determined before application of the pressure and after 24 h of culture without a pressure difference between the anterior and the posterior corneal surfaces. Pressure differences of 160 and 80 mmHg maintained for 8 and 72 h did not lead to decreased endothelial cell densities.