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DECONTAMINATION OF CADAVER CORNEAS
Author(s) -
SPERLING STEFFEN,
SØRENSEN INGER G.
Publication year - 1981
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1981.tb06720.x
Subject(s) - cadaver , medicine , pipette , surgery , chemistry
Bacterial contamination of human corneas, in vivo and post mortem, was investigated. Bacterial samples were obtained by pressing small concave agar plates directly against the corneas. More colony forming units (C. F. U.) occurred in samples obtained from patients in general anaesthesia than in samples obtained from healthy persons in local anaesthesia. The largest number of C.F.U. occurred post mortem. The number of C.F.U. obtained from cadaver corneas was not correlated to post mortem time. Rinsing with water removed 86% of the C. F. U. on cadaver corneas. Rinsing with water for 30 sec followed by 2 min treatment with 0.5% polyvinylpyrrolidone‐iodine (PVP‐I) and repeated rinsing removed 98% of the C. F. U. Epithelial or stromal lesions were not found after 2 min of exposure to 0.5% PVP‐I.