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HYDROGEN PEROXIDE AND CORNEAL ENDOTHELIUM
Author(s) -
HULL DAVID S.,
CSUKAS STEVE,
GREEN KEITH,
LIVINGSTON VELDA
Publication year - 1981
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1981.tb03006.x
Subject(s) - hydrogen peroxide , cornea , corneal endothelium , toxicity , chemistry , endothelium , ascorbic acid , swelling , glutathione , catalase , perfusion , rose bengal , antioxidant , endothelial stem cell , cell damage , biochemistry , pharmacology , medicine , ophthalmology , in vitro , endocrinology , pathology , enzyme , food science , organic chemistry
Because of recent evidence of low levels of hydrogen peroxide in the aqueous humor, studies were performed to determine levels of corneal endothelial toxicity as well as factors modifying toxicity. Perfusion of cornea endothelial cells for 3 h with varying concentrations of hydrogen peroxide demonstrated a threshold of toxicity at a nominal concentration between 0.3 and 0.5 mM H 2 O 2 . The toxic effect resulted in rapid corneal swelling as well as disruption of endothelial cell cytoplasm and organelles. Both the physiologic and anatomic toxic effects of 0.5 mM H 2 O 2 could be blocked with 5400 U/ml catalase. Exposure of corneas to 20 mM H 2 O 2 for 10 min in the presence of EDTA ‐ Fe +3 resulted in an enhancement of corneal swelling rate more rapid than that which resulted from a 10 min exposure to 20 mM H 2 O 2 alone. Neither the presence of ascorbic acid nor the absence of glutathione and adenosine had an effect on the cornea swelling rate which occurred during a 3 h perfusion of endothelium with 0.3 mM H 2 O 2 . Chelated iron had no effect on the corneal swelling rate induced by phototactivation of rose bengal presensitized cornea endothelial cells.