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ON THE FORMATION OF ELSCHNIG'S PEARLS A TISSUE CULTURE STUDY OF REGENERATING RAT LENS EPITHELIUM
Author(s) -
FAGERHOLM P.
Publication year - 1980
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1980.tb08323.x
Subject(s) - vacuole , lens (geology) , extracellular , lens fiber , epithelium , organ culture , anatomy , electron microscope , microbiology and biotechnology , chemistry , biology , pathology , cytoplasm , optics , medicine , biochemistry , in vitro , paleontology , physics
Rat lens epithelial cells were allowed to regenerate on the lens capsule in tissue culture. The major part of the lens fibers had been removed. After a month spherical bodies appeared, similar in appearance to Elschnig's pearls. Electron microscopical examination revealed that these spheres consisted of large extracellular vacuoles, and phase contrast examination revealed a continuous change in appearance of these spheres. The mechanism behind the tendency to form extracellular vacuoles probably include an increased extrusion of sodium and water from osmotically stressed lens cells.