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TOXICITY OF DIMETHYLSULFOXIDE (DMSO) TO HUMAN CORNEAL ENDOTHELIUM IN VITRO
Author(s) -
SPERLING STEFFEN,
LARSEN INGER G.
Publication year - 1979
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1979.tb01856.x
Subject(s) - in vitro , toxicity , corneal endothelium , chemistry , dimethyl sulfoxide , organ culture , dextran , cell , mole , albumin , endothelium , cell damage , bovine serum albumin , microbiology and biotechnology , andrology , biochemistry , biology , medicine , endocrinology , organic chemistry
Human cadaver corneas were obtained 10–12 h after clinical death, incubated in organ culture for 20–28 h and exposed to 2, 4, 6, 8, 6, 4, and 2% DMSO successively for 10 min at each concentration. Endothelial cell damage was recognized by morphological alterations during reformation of a coherent cell sheat after expulsion of damaged cells. DMSO was found to cause latent cell damage at 31°C. No cell damage was found at 4°C when DMSO was dissolved in minimum essential medium (Eagle) plus Dextran or when DMSO was dissolved in human albumin plus sucrose. DMSO was found to be toxic at 4°C in pure serum and in Dulbecco's medium.