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IN VITRO PRODUCTION OF STEROID CATARACT IN BOVINE LENS
Author(s) -
MAYMAN CHAIM I.,
MILLER DAVID,
TIJERINA M. L.
Publication year - 1979
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1979.tb00545.x
Subject(s) - ouabain , dexamethasone , incubation , in vitro , chemistry , phosphate , steroid , endocrinology , incubation period , medicine , enzyme , biochemistry , biology , sodium , organic chemistry , hormone
Fresh calf lenses incubated in nutritive media containing dexamethasone phosphate or ouabain in concentrations ranging from 1 × 10 −4 M to 1 × 10 −8 M developed cortical opacification and showed significant inhibition of Na + ‐K + ATP'ase activity. Over a 3‐day incubation period the decrease in Na + ‐K + ATP'ase activity correlated well with the observed decrease in light transmission. The degree of enzyme inhibition and decrease in light transmission varied directly with the concentration of dexamethasone phosphate and ouabain, with significant changes observed at ‘physiologic’ and ‘pharmacologic’ concentrations of these agents. Lenses incubated for 4 days in dexamethasone phosphate or ouabain showed substantial increases in water content as well as an increase in Na + and a decrease in K + concentration. These data suggest that inhibition of the cation pump may play a significant role in the formation of steroid cataract in vitro .