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IN VITRO PRODUCTION OF STEROID CATARACT IN BOVINE LENS
Author(s) -
MILLER DAVID,
TIJERINA M. L.,
MAYMAN CHAIM
Publication year - 1979
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1979.tb00544.x
Subject(s) - dexamethasone , ouabain , incubation , lens (geology) , incubation period , chemistry , in vitro , steroid , biology , endocrinology , biochemistry , sodium , paleontology , organic chemistry , hormone
Fresh calf lenses were incubated in nutritive media, to which was added ouabain and dexamethasone phosphate at concentrations of 1 × 10 ‐4 M. At the end of a three day incubation period, cortical opacification developed in the control series of lenses as well as those lenses incubated in both ouabain and dexamethasone phosphate. Using a light transmission device which quantitates lens opacification, it was noted that dexamethasone produced a level of cortical opacification significantly greater than that of the control series. Ouabain produced a level of cortical opacification statistically identical to that produced by the dexamethasone. It is suggested that the aforementioned light transmission device is an accurate and reproducible method of quantitating cataractous opacification.