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IMMUNOELECTROPHORETIC DETERMINATION OF TEAR FLUID PROTEINS COLLECTED BY THE SCHIRMER I TEST
Author(s) -
PRAUSE J. U.
Publication year - 1979
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1979.tb00526.x
Subject(s) - chromatography , schirmer test , tears , ophthalmology , detection limit , chemistry , medicine , immunology , dry eyes
Using reservoirs containing 50 μl of antigen solution in combination with a micro‐modification of the electroimmunoassay a detection limit of 0.05 ng applied in 400 μl corresponding to 0.125 ng/ml has been achieved. Tear fluid eluate from Schirmer paper strips has been tested in the assay. The eluation procedure failed to extract all tear proteins as 32 ng were left in the paper. Despite the loss, experiments have shown the method to be suitable for immunological determination of tear proteins colleced by the Shirmer I test.