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POLYSACCHARIDE COATING OF HUMAN CORNEAL ENDOTHELIUM
Author(s) -
SCHRøDER HENRIK DAA,
SPERLING STEFFEN
Publication year - 1977
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1977.tb08281.x
Subject(s) - osmium tetroxide , corneal endothelium , polysaccharide , fixation (population genetics) , layer (electronics) , staining , endothelium , coating , osmium , ruthenium red , chemistry , materials science , electron microscope , biomedical engineering , pathology , ruthenium , biochemistry , medicine , nanotechnology , optics , organic chemistry , physics , gene , calcium , catalysis
Electron microscopy revealed the presence of a 600–1500 å thick layer of polysaccharide on the surface of human corneal endothelial cells. The surface layer was visualized by combined fixation and staining in a mixture of ruthenium red and osmium tetroxide. The coating material was stable for at least 39 h post mortem and was retained on disintegrating cells.