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FACTORS AFFECTING THE SPONTANEOUS RELEASE OF [ 3 H]GLYCINE FROM RABBIT RETINA
Author(s) -
BAUER BIRGITTA
Publication year - 1977
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1977.tb05674.x
Subject(s) - glycine , ouabain , amino acid , efflux , retina , glutamic acid , alanine , chemistry , retinal , biophysics , biochemistry , taurine , biology , sodium , neuroscience , organic chemistry
The efflux of [ 3 H]glycine was studied in superfused rabbit retina in the presence of various amino acids, ouabain, or high K + or low Ca 2+ concentrations in the superfusion medium. Unlabelled glycine evoked an accelerated efflux as did the structurally similar neutral α‐amino acids. β‐alanine and GABA were ineffective. The results demonstrate a homo‐exchange of glycine, and a heteroexchange with the neutral α‐amino acids. A low concentration of glutamic acid (10 ‐5 m) will release glycine from the retina. This is an ATPase dependent process which is partially blocked by a high Mg 2+ /Ca 2+ ratio and which may be related to a retinal transmitter function of glutamic acid. A high concentration of K + or the presence of ouabain in the superfusing medium greatly increases the rate at which glycine is lost from the retina.