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A LIGHT AND ELECTRON MICROSCOPIC STUDY OF THE PERMEABILITY OF THE RABBIT IRIS VESSELS TO HORSERADISH PEROXIDASE IN EXPERIMENTAL UVEITIS
Author(s) -
PEDERSEN OLAV ØYVIND
Publication year - 1974
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1974.tb01103.x
Subject(s) - horseradish peroxidase , peroxidase , vascular permeability , chemistry , iris (biosensor) , uveitis , endothelium , enucleation , anatomy , electron microscope , ophthalmology , pathology , medicine , biochemistry , surgery , enzyme , optics , computer security , computer science , biometrics , physics
Experimental uveitis has been produced in two groups of albino rabbits by a single intravitreal injection of antigen. The animals in group I were immunized by injection of 10 mg of human serum albumin, whereas those belonging to group II received 50 mg. To study the blood‐aqueous barrier to proteins in the iris vessels of these eyes, horseradish peroxidase has been used as a protein tracer by light and electron microscopy. The tracer was injected intravenously at different time intervals (1–30 min) before enucleation. In group I, no leakage of peroxidase from the iris vessels was demonstrated in any eyes except one. The diffusion of peroxidase was halted by the zonulae occludentes of the vascular endothelium, and there was no evidence of vesicular transport of peroxidase through the endothelial cells. In the more heavy inflamed eyes belonging to group II, a prominent inflammatory cell infiltration was found in the iris. In these eyes leakage of peroxidase through the interendothelial clefts of the iris vessels was demonstrated.