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METHOD OF TESTING DYES FOR VITAL STAINING OF CORNEA AND CONJUNCTIVA
Author(s) -
NORN M. S.
Publication year - 1972
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.1972.tb06620.x
Subject(s) - vital stain , staining , stain , conjunctiva , cornea , pathology , rose bengal , eosin , medicine , chemistry , ophthalmology , organic chemistry
Lines are given for testing dyes intended for use in vital staining of the cornea and conjunctiva, especially dyes having properties different from those of the well‐known fluorescein and rose bengal. A priority list for dye testing has been made comprising six items:1) Water‐solubility (a 1% solution in non‐irritant vehicle is desirable). 2) Toxicity tests (dyes should cause no smarting pain nor effect permanent tatooing). 3) Microscopy of the mucous thread in the inferior conjunctival fornix (possible staining of mucous fibrils, debris in the mucous thread vacuoles, different cell types, nucleus, cytoplasm, mitochondria, etc.). 4) Clinical vital staining of normal eyes, keratitis, etc. 5) Double staining with a known vital stain (successive staining or use of a dye mixture) of a clinical material examined in slit lamp or microscope. 6) Conclusion and entering in a diagram of the vital stains tested so far.