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Topoisomerase I amplification in melanoma is associated with more advanced tumours and poor prognosis
Author(s) -
Ryan Denise,
Rafferty Mairin,
Hegarty Shauna,
O’Leary Patrick,
Faller William,
Gremel Gabriela,
Bergqvist Michael,
Agnarsdottir Margret,
Strömberg Sara,
Kampf Caroline,
Pontén Fredrik,
Millikan Robert C.,
Dervan Peter A.,
Gallagher William M.
Publication year - 2010
Publication title -
pigment cell and melanoma research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.618
H-Index - 105
eISSN - 1755-148X
pISSN - 1755-1471
DOI - 10.1111/j.1755-148x.2010.00720.x
Subject(s) - comparative genomic hybridization , melanoma , fluorescence in situ hybridization , biology , tissue microarray , cancer research , fish <actinopterygii> , in situ hybridization , genetics , cancer , gene , genome , chromosome , gene expression , fishery
Summary In this study, we used array‐comparative genomic hybridization (aCGH) and fluorescent in situ hybridization (FISH) to examine genetic aberrations in melanoma cell lines and tissues. Array‐comparative genomic hybridization revealed that the most frequent genetic changes found in melanoma cell lines were amplifications on chromosomes 7p and 20q, along with disruptions on Chr 9, 10, 11, 12, 22 and Y. Validation of the results using FISH on tissue microarrays (TMAs) identified TOP1 as being amplified in melanoma tissues. TOP1 amplification was detected in a high percentage (33%) of tumours and was associated with thicker, aggressive tumours. These results show that TOP1 amplification is associated with advanced tumours and poor prognosis in melanoma. These observations open the possibility that TOP1 ‐targeted therapeutics may be of benefit in a particular subgroup of advanced stage melanoma patients.