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MC1R stimulation by α‐MSH induces catalase and promotes its re‐distribution to the cell periphery and dendrites
Author(s) -
Maresca Vittoria,
Flori Enrica,
Bellei Barbara,
Aspite Nicaela,
Kovacs Daniela,
Picardo Mauro
Publication year - 2010
Publication title -
pigment cell and melanoma research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.618
H-Index - 105
eISSN - 1755-148X
pISSN - 1755-1471
DOI - 10.1111/j.1755-148x.2010.00673.x
Subject(s) - catalase , microphthalmia associated transcription factor , stimulation , melanocortin 1 receptor , melanocyte stimulating hormone , microbiology and biotechnology , biology , endocrinology , transcription factor , medicine , receptor , hormone , biochemistry , oxidative stress , phenotype , gene
Summary We demonstrated a direct correlation between melanogenic and catalase activities on in vitro and ex vivo models. Here, we investigated whether the stimulation of Melanocortin‐1 Receptor (MC1R) could influence catalase expression, activity and cellular localization. For this purpose, we treated B16‐F0 melanoma cells with α‐Melanocyte Stimulating Hormone (α‐MSH) and we showed a rapid induction of catalase through a cAMP/PKA‐dependent, microphthalmia‐associated transcription factor (MITF) independent mechanism, acting at post‐transcriptional level. Moreover, α‐MSH promoted a partial re‐distribution of catalase to the cell periphery and dendrites. This work strengthens the correlation between melanogenesis and anti‐oxidants, demonstrating the induction of catalase in response to a melanogenic stimulation, such as α‐MSH‐dependent MC1R activation. Moreover, this study highlights catalase regulatory mechanisms poorly known, and attributes to α‐MSH a protective role in defending melanocytes, and possibly keratinocytes, not only on the basis of its pigmentary action, but also for its capacity to stimulate a quick anti‐oxidant defence.