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Coelomic fluid: a noninvasive source of DNA in earthworms
Author(s) -
MINAMIYA YUKIO,
OHGA KYOHEI,
HAYAKAWA HIROSHI,
ITO KATSURA,
FUKUDA TATSUYA
Publication year - 2011
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2011.03003.x
Subject(s) - biology , coelom , earthworm , dna , cytochrome c oxidase , mitochondrial dna , genbank , anatomy , microbiology and biotechnology , biochemistry , ecology , enzyme , gene
To investigate whether coelomic fluid secreted by earthworms can be a noninvasive source of DNA, we amplified and sequenced DNA extracted from the coelomic fluid and muscle tissue of eight worms. The sequences obtained using DNA extracted from both sources were identical. All cytochrome c oxidase subunit I (COI) mitochondrial DNA sequences, including those retrieved from GenBank, formed a monophyletic group of Metaphire sieboldi . The results indicate that we successfully extracted total DNA from coelomic fluid secreted by earthworm.