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Nested polymerase chain reaction–based detection of Dothistroma septosporum , red band needle blight of pine, a tool in support of phytosanitary regimes
Author(s) -
LANGRELL STEPHEN R. H.
Publication year - 2011
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2011.02996.x
Subject(s) - biology , phytosanitary certification , nested polymerase chain reaction , polymerase chain reaction , pathogen , blight , primer (cosmetics) , pinus <genus> , biosecurity , botany , identification (biology) , horticulture , microbiology and biotechnology , genetics , ecology , gene , chemistry , organic chemistry
Red band needle blight is one of the most important foliar diseases of Pinus species and is of increasing international forest health and biosecurity concern. To provide a rapid identification technique for this pathogen in support of official control measures, a nested polymerase chain reaction–based diagnostic assay that employs species‐specific primer sets has been developed. The assay is able to detect the presence of the pathogen direct from pine needles, irrespective of host species, to within 10 fg of target DNA, the equivalent of approximately 2–3 ascospores or hyphael cells.