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Coupling non‐destructive DNA extraction and voucher retrieval for small soft‐bodied Arthropods in a high‐throughput context: the example of Collembola
Author(s) -
PORCO DAVID,
ROUGERIE RODOLPHE,
DEHARVENG LOUIS,
HEBERT PAUL
Publication year - 2010
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2010.2839.x
Subject(s) - biology , lysis , voucher , context (archaeology) , dna extraction , clearing , throughput , extraction (chemistry) , dna , biological system , toxicology , polymerase chain reaction , chromatography , genetics , biochemistry , accounting , computer science , gene , paleontology , telecommunications , chemistry , finance , economics , wireless , business
Abstract Here, we describe a simple method adapted for high‐throughput protocols allowing voucher specimen recovery for Collembola and by extension for other soft‐bodied small arthropods. A standard extraction protocol was tested to examine the effects of lysis duration (1, 2, 4, 12 h) on DNA concentration, amplification success and specimen condition. Good quality DNA was obtained after 1 h of lysis, while voucher condition was fine for up to 12 h. The lysis step substantially shortens the clearing process necessary for morphological examination.