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Assembling a dual purpose TaqMan‐based panel of single‐nucleotide polymorphism markers in rainbow trout and steelhead ( Oncorhynchus mykiss ) for association mapping and population genetics analysis
Author(s) -
HANSEN METTE H. H.,
YOUNG SEWALL,
JØRGENSEN HANNE B. H.,
PASCAL CARITA,
HENRYON MARK,
SEEB JAMES
Publication year - 2011
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2010.02978.x
Subject(s) - taqman , rainbow trout , biology , genotyping , genetics , single nucleotide polymorphism , snp genotyping , computational biology , genotype , gene , polymerase chain reaction , fishery , fish <actinopterygii>
We establish a TaqMan‐based assay panel for genotyping single‐nucleotide polymorphisms in rainbow trout and steelhead ( Oncorhynchus mykiss ). We develop 22 novel single‐nucleotide polymorphism markers based on new steelhead sequence data and on assays from sister taxa. Additionally, we adapt 154 previously developed markers to the TaqMan platform. At the beginning of this study, 59 SNPs with TaqMan assays were available to the scientific community. By adding 176 additional TaqMan assays to this number, we greatly expand the biological applications of TaqMan genotyping within both population genetics and quantitative genetics.