Premium
Development of novel microsatellite markers to identify the different invasive lineages in the Corbicula complex and to assess androgenesis
Author(s) -
PIGNEUR L. M.,
RISTERUCCI A. M.,
DAUCHOT N.,
LI X.,
VAN DONINCK K.
Publication year - 2011
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2010.02963.x
Subject(s) - biology , microsatellite , locus (genetics) , genetics , evolutionary biology , genetic marker , mitochondrial dna , phylogenetics , gene , allele
Reliable markers are needed to identify the lineages in the invasive clam genus Corbicula . Previous studies have demonstrated that mitochondrial (mt) DNA poorly resolves Corbicula phylogeny, owing to its androgenetic reproductive mode. Moreover, hybridization and mitochondrial/nuclear mismatches occur. We developed the first eleven polymorphic markers to detect these phenomena and to investigate the nuclear identity of Corbicula populations. These microsatellite loci revealed three main lineages in Western Europe. One locus allowed rapid discrimination of these three lineages on agarose gel, saving time and money. Moreover, the eleven markers were successfully cross‐amplified in the invasive Corbicula lineages found in North America.