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A noninvasive, direct real‐time PCR method for sex determination in multiple avian species
Author(s) -
BRUBAKER JESSICA L.,
KAROUNARENIER NATALIE K.,
CHEN YU,
JENKO KATHRYN,
SPRAGUE DANIEL T.,
HENRY PAULA F. P.
Publication year - 2011
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2010.02951.x
Subject(s) - biology , computational biology , real time polymerase chain reaction , zoology , evolutionary biology , genetics , gene
Polymerase chain reaction (PCR)‐based methods to determine the sex of birds are well established and have seen few modifications since they were first introduced in the 1990s. Although these methods allowed for sex determination in species that were previously difficult to analyse, they were not conducive to high‐throughput analysis because of the laboriousness of DNA extraction and gel electrophoresis. We developed a high‐throughput real‐time PCR‐based method for analysis of sex in birds, which uses noninvasive sample collection and avoids DNA extraction and gel electrophoresis.