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An ARMS‐based technique for sex determination of red panda ( Ailurus fulgens )
Author(s) -
LI YUZHI,
XU XIAO,
ZHANG LIANG,
ZHANG ZHIHE,
SHEN FUJUN,
ZHANG WENPING,
YUE BISONG
Publication year - 2011
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2010.02934.x
Subject(s) - biology , sexing , amplicon , genetics , dna , agarose gel electrophoresis , microbiology and biotechnology , exon , gene , polymerase chain reaction
Molecular sexing is a key component in the investigation of wild populations. In this study, we developed a fast, accurate and reliable amplification refractory mutation system (ARMS) technique for sex determination of red panda based on the exon 4 of the ZFX/ZFY gene. The amplicons were distinguished simply by agarose gel electrophoresis, exhibiting one fragment in females (X: 300 bp) and two in males (X: 300 bp, Y: 166 bp). Robustness of this ARMS system was confirmed by testing both 43 captive red pandas using DNA samples with known‐sex and 10 wild red pandas using faecal DNA samples with unknown sex.