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A primer set to determine sex in the small Indian mongoose, Herpestes auropunctatus
Author(s) -
MURATA C.,
OGURA G.,
KUROIWA A.
Publication year - 2011
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2010.02931.x
Subject(s) - biology , sexing , mongoose , primer (cosmetics) , genomic dna , genetics , polymerase chain reaction , w chromosome , microbiology and biotechnology , dna , zoology , gene , chemistry , organic chemistry , karyotype , chromosome
To enable the accurate sexing of individuals of introduced populations of the small Indian mongoose, Herpestes auropunctatus , we designed a primer set for the amplification of the sex‐specific fragments EIF2S3Y and EIF2S3X . Using this primer set, the expected amplification products were obtained for all samples of genomic DNA tested: males yielded two bands and females a single band. Sequencing of each PCR product confirmed that the 769‐bp fragment amplified from DNA samples of both sexes was derived from EIF2S3X, whereas the 546‐bp fragment amplified only from male DNA samples was derived from EIF2S3Y . The results indicated that this primer set is useful for sex identification in this species.